Jeffrey Chen

About

I am a molecular microbiologist engaged in tuberculosis and mycobacteria research. The work in my lab is focused on: 1) Understanding the transmission and pathogenesis of mycobacterial infections. 2) Developing large-animal models of tuberculosis transmission and infection. 3) Developing improved tuberculosis vaccines and drugs. 4) BCG vaccine mediated trained immunity in humans and livestock species.

Work

University of Saskatchewan
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Principal Research Scientist / Principal Investigator

Canada

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École Polytechnique Fédérale de Lausanne
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CIHR and Canadian Thoracic Society post-doctoral fellow

Switzerland

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Education

University of Toronto
Canada

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PhD

Dalhousie University
Canada

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BSc (Honours)

Publications

Assembly of the Mycobacterium tuberculosis type VII ESX-1 secretion system in Mycobacterium smegmatis identifies a new transcriptional activator of esx-1 genes and a novel TB vaccine

Published by

Microbiology Spectrum

Summary

journal-article

The Parkinson's drug benztropine possesses histamine receptor 1-dependent host-directed antimicrobial activity against Mycobacterium tuberculosis.

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npj antimicrobials and resistance

Summary

journal-article

Optimization and validation of ELISAs for interferon-gamma determination in bison.

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Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Summary

journal-article

Orally administered live BCG and heat-inactivated Mycobacterium bovis protect bison against experimental bovine tuberculosis.

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Scientific reports

Summary

journal-article

A recombinant bacterial strain as tuberculosis vaccine candidate and methods of producing thereof.

Summary

patent

Mycobacterium tuberculosis EspK has active but distinct roles in the secretion of EsxA and EspB

Published by

Journal of Bacteriology

Summary

journal-article

Mycobacterium tuberculosis EspK Has Active but Distinct Roles in the Secretion of EsxA and EspB

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Journal of Bacteriology

Summary

journal-article

Pyrazole and Triazole Derivatives as Mycobacterium tuberculosis UDP-Galactopyranose Inhibitors

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Pharmaceuticals

Summary

journal-article

Domestic pigs experimentally infected with Mycobacterium bovis and Mycobacterium tuberculosis exhibit different disease outcomes

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Tuberculosis

Summary

journal-article

Protein Synthesis and Degradation Inhibitors Potently Block Mycobacterium tuberculosis type-7 Secretion System ESX-1 Activity

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ACS Infectious Diseases

Summary

journal-article

A SNP in the Cache 1 Signaling Domain of Diguanylate Cyclase STM1987 Leads to Increased in vivo Fitness of Invasive Salmonella Strains.

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Infection and immunity

Summary

journal-article

A SNP in the Cache 1 Signaling Domain of Diguanylate Cyclase STM1987 Leads to Increased In Vivo Fitness of Invasive Salmonella Strains

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Infection and Immunity

Summary

journal-article

Poor stimulation of bovine dendritic cells by Mycobacterium bovis culture supernatant and surface extract is associated with decreased activation of ERK and NF-κB and higher expression of SOCS1 and 3.

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Innate immunity

Summary

journal-article

Poor stimulation of bovine dendritic cells by Mycobacterium bovis culture supernatant and surface extract is associated with decreased activation of ERK and NF-κB and higher expression of SOCS1 and 3

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Innate Immunity

Summary

journal-article

Novel approaches to tuberculosis vaccine development using novel animal models

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The 5th International One Health Congress

Summary

conference-paper

Suppression of the ESX-1 system – mechanisms, consequences & implications

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American Society for Microbiology Conference on Tuberculosis – Past, Present and Future

Summary

conference-paper

Mycosins of the Mycobacterial Type VII ESX Secretion System: the Glue That Holds the Party Together.

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mBio

Summary

journal-article

Chemical Genetics of the M. tuberculosis ESX-1 Secretion System – from anti-virulence drugs to gene-function discovery

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Department of Microbiology and Immunology Seminar Series - College of Medicine, University of Saskatchewan

Summary

lecture-speech

Mycobacterium tuberculosis EspD is a secreted virulence modulator

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Canadian Society of Microbiologists 65th Annual Conference

Summary

conference-paper

The Type-7 ESX-1 Secretion System and Aerobiology of M. tuberculosis – prospective targets for TB therapy

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Department of Chemistry and Biochemistry, University of Regina

Summary

lecture-speech

Anticytolytic screen identifies inhibitors of mycobacterial virulence protein secretion.

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Cell host & microbe

Summary

journal-article

EspI regulates the ESX-1 secretion system in response to ATP levels in Mycobacterium tuberculosis.

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Molecular microbiology

Summary

journal-article

The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron-sulfur cluster biogenesis and oxidative stress defence.

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The Biochemical journal

Summary

journal-article

Towards New Tools to Fight Tuberculosis

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Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan

Summary

lecture-speech

Understanding and targeting Mycobacterium tuberculosis virulence and transmission to combat TB

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Institut National de la Recherche Scientifique - Institut Armand-Frappier

Summary

lecture-speech

Phenotypic profiling of Mycobacterium tuberculosis EspA point mutants reveals that blockage of ESAT-6 and CFP-10 secretion in vitro does not always correlate with attenuation of virulence.

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Journal of bacteriology

Summary

journal-article

Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence.

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Molecular microbiology

Summary

journal-article

Differential characteristics and virulence of Mycobacterium tuberculosis espACD and ESX-1 mutants – revisiting the role of EspA

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EMBO conference series - Tuberculosis 2012: Biology, Pathogenesis and Intervention strategies at the Institut Pasteur

Summary

conference-poster

A point mutation in cycA partially contributes to the D-cycloserine resistance trait of Mycobacterium bovis BCG vaccine strains.

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PloS one

Summary

journal-article

Virulence regulator EspR of Mycobacterium tuberculosis is a nucleoid-associated protein.

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PLoS pathogens

Summary

journal-article

EspD is critical for the virulence-mediating ESX-1 secretion system in Mycobacterium tuberculosis.

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Journal of bacteriology

Summary

journal-article

Biochemical and immunological characterization of EspD, an atypical ESX-1 secretion-associated protein

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Eighth International Conference on the Pathogenesis of Mycobacterial Infections

Summary

conference-poster

Mutational Analyses of EspA and Effects on the Mycobacterium tuberculosis ESX-1 Virulence Protein Secretion System

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FEMS 2011, 4th Congress of European Microbiologists

Summary

conference-poster

EspD plays a critical role in the Mycobacterium tuberculosis ESX-1 secretion system

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Keystone Symposia (Joint Meeting) - Tuberculosis: Immunology, Cell Biology and Novel Vaccination Strategies, and Mycobacteria: Physiology, Metabolism and Pathogenesis - Back to the Basics

Summary

conference-poster

ESAT-6 secretion-independent impact of ESX-1 genes espF and espG1 on virulence of Mycobacterium tuberculosis.

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The Journal of infectious diseases

Summary

journal-article

Sigma factor F does not prevent rifampin inhibition of RNA polymerase or cause rifampin tolerance in Mycobacterium tuberculosis.

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Journal of bacteriology

Summary

journal-article

Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

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Drug Discovery Today: Disease Mechanisms

Summary

journal-article

Tuberculosis vaccines including recombinant BCG strains expressing alanine dehydrogenase, serine dehydratase and/or glutamine synthetase

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United States Patent and Trademark Office, no. 7,611,720

Summary

patent

Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein.

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Nucleic acids research

Summary

journal-article

Differential productions of lipid virulence factors among BCG vaccine strains and implications on BCG safety.

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Vaccine

Summary

journal-article

Identification of the lipooligosaccharide biosynthetic gene cluster from Mycobacterium marinum.

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Molecular microbiology

Summary

journal-article

PimF, a mannosyltransferase of mycobacteria, is involved in the biosynthesis of phosphatidylinositol mannosides and lipoarabinomannan.

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The Journal of biological chemistry

Summary

journal-article

Mycobacterium bovis BCG vaccines exhibit defects in alanine and serine catabolism.

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Infection and immunity

Summary

journal-article

Site-directed mutagenesis of acyl carrier protein (ACP) reveals amino acid residues involved in ACP structure and acyl-ACP synthetase activity.

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The Journal of biological chemistry

Summary

journal-article