About

Highly accomplished Professor with over 40 years of expertise in ligand-nucleic acid interactions, transcriptional regulation, and methods development, specializing in biochemistry and molecular biology. Proven leader in securing substantial grant funding and driving innovative research, particularly in extremophile organisms and DNA-binding mechanisms. Dedicated to fostering scientific inquiry and mentoring the next generation of researchers in primarily undergraduate institutions.

Michael W. Van Dyke

Professor of Chemistry and Biochemistry
Kennesaw, US.

Education

The Rockefeller University
New York, NY, United States of America

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Postdoctoral Fellow

Biochemistry and Molecular Biology

California Institute of Technology
Pasadena, CA, United States of America

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PhD

Chemistry and Chemical Engineering

Work

Kennesaw State University
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Professor (Chemistry and Biochemistry)

Kennesaw, GA, US

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Summary

Led advanced research and taught chemistry and biochemistry, focusing on ligand-nucleic acid interactions and transcriptional regulation while securing significant external funding.

Highlights

Secured over $1.5M in competitive grants from institutions like the National Institute of General Medical Sciences and the National Science Foundation, funding critical research into transcription factor discovery and nucleic acid analysis.

Directed cutting-edge research projects on transcription factor discovery in extremophile organisms (Thermus thermophilus) and opportunistic pathogens (Pseudomonas aeruginosa), advancing understanding of gene regulation.

Developed and implemented novel methods for studying ligand-nucleic acid interactions, including the use of biolayer interferometry and advanced spectroscopic techniques, enhancing research capabilities at undergraduate institutions.

Published numerous peer-reviewed articles in high-impact journals such as Molecular Microbiology and Journal of Biological Chemistry, contributing to over 79 total publications in the field.

Mentored undergraduate researchers, guiding them through complex biochemical experiments and fostering their development in scientific methodology and critical thinking.

Western Carolina University
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Associate Professor (Chemistry and Physics)

Cullowhee, NC, US

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Summary

Contributed to the Chemistry and Physics departments through teaching and research, focusing on molecular interactions and methods development.

Highlights

Developed and delivered comprehensive curriculum in chemistry and physics, engaging students in complex scientific principles and laboratory techniques.

Conducted independent research exploring molecular mechanisms, building upon a strong foundation in ligand-nucleic acid interactions and transcriptional regulation.

Contributed to the academic community through departmental service and collaborative initiatives, enhancing the research and educational environment.

Mentored junior researchers and students, fostering their growth in scientific inquiry and preparing them for advanced studies or careers in science.

The University of Texas Graduate School of Biomedical Sciences
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Member

Houston, TX, US

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Summary

Contributed as a key member to biomedical research initiatives, specializing in molecular and cellular oncology.

Highlights

Played a pivotal role in research on small molecule-DNA interactions and general eukaryotic transcription mechanisms, contributing to significant advancements in the field over a 21-year tenure.

Applied and developed combinatorial selection methods, including REPSA, to identify preferred DNA-binding sequences for various proteins and ligands, enhancing drug discovery and understanding of gene regulation.

Published extensively on topics such as nucleolin protein interactions, ribosome preservation during quiescence, and the molecular mechanisms of histone deacetylase regulation, contributing to over 50 publications during this period.

Collaborated with interdisciplinary teams on projects related to tumor biology and molecular oncology, translating complex biochemical findings into clinically relevant insights.

Mentored graduate students and postdoctoral fellows, guiding their research projects and contributing to their professional development in biomedical sciences.

The University of Texas MD Anderson Cancer Center
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Assistant Biologist / Associate Biologist (Tumor Biology / Molecular and Cellular Oncology)

Houston, TX, US

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Summary

Conducted advanced biological research within tumor biology and molecular/cellular oncology, progressing from Assistant to Associate Biologist.

Highlights

Initiated and executed independent research projects focused on the molecular mechanisms underlying tumor biology and cellular oncology, contributing to the institution's mission.

Utilized and refined techniques such as immobilized metal affinity chromatography and G*G multiplex nucleic acids to investigate complex biological pathways relevant to cancer research.

Published critical findings on topics including parthenolide's role in p53 activation and histone deacetylase depletion, advancing understanding of cancer therapeutics.

Collaborated effectively with a diverse team of scientists and clinicians, contributing specialized expertise in molecular interactions and gene regulation to large-scale research initiatives.

Maintained rigorous laboratory standards and contributed to the development of new experimental protocols, ensuring high-quality and reproducible research outcomes.

The Rockefeller University
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Senior Research Scientist (Biochemistry and Molecular Biology)

New York, NY, US

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Summary

Led advanced research in biochemistry and molecular biology, contributing to foundational discoveries in gene regulation and DNA interactions.

Highlights

Conducted high-impact research on DNA-binding and transcriptional properties of human transcription factor TFIID, contributing to a seminal publication in Molecular and Cellular Biology.

Investigated the physical analysis of transcription preinitiation complex assembly on class II gene promoters, publishing findings in Science.

Explored the binding of multiple proteins to VA RNA genes of adenovirus Type 2, contributing to a deeper understanding of viral gene expression.

Utilized advanced molecular biology techniques to characterize complex protein-DNA interactions, setting the stage for future research in gene regulation.

Collaborated with leading scientists in a world-renowned research environment, contributing to the institution's legacy of scientific excellence.

Awards

Transcription Factor Discovery in the Opportunistic Pathogen Pseudomonas aeruginosa

Awarded By

National Institute of General Medical Sciences (Bethesda)

Awarded R16GM154191 grant to investigate transcription factor discovery in Pseudomonas aeruginosa, a project spanning July 2024 to June 2028.

RUI: Transcription Factor Discovery in the Extremophile Thermus Thermophilus

Awarded By

Directorate for Biological Sciences (Arlington)

Received grant (2041202) for research on transcription factor discovery in Thermus thermophilus, active from July 2021 to June 2024.

RUI: Transcription Factor Discovery in the Extremophile Thermus Thermophilus

Awarded By

Directorate for Biological Sciences (Arlington)

Awarded grant (1714778) for transcription factor discovery in Thermus thermophilus, spanning July 2017 to June 2021.

RUI: Refractory Cleavage Sites to Elucidate Type IIS Restriction Endonuclease Mechanisms

Awarded By

Directorate for Mathematical & Physical Sciences

Secured grant (1709263) to elucidate mechanisms of Type IIS restriction endonucleases through refractory cleavage sites, active from September 2017 to August 2019.

MRI: Acquisition of a Nucleic Acid Analyzer for Research and Teaching

Awarded By

National Science Foundation - Directorate for Biological Sciences (Arlington)

Obtained grant (1624654) for the acquisition of a nucleic acid analyzer, enhancing research and teaching capabilities from August 2016 to July 2019.

DNA-Binding Protein Discovery by the Combinatorial Method REPSA

Awarded By

National Institute of General Medical Sciences (Bethesda)

Received grant (R15GM104833) to develop and apply the REPSA combinatorial method for DNA-binding protein discovery, active from July 2013 to June 2017.

MRI: Acquisition of a Spectropolarimeter

Awarded By

Directorate for Biological Sciences (Arlington)

Secured grant (1337781) for the acquisition of a spectropolarimeter, supporting research from October 2013 to September 2016.

Publications

Manganese-dependent transcription regulation by MntR and PerR in Thermus thermophilusHB8

Published by

Molecular Microbiology

Summary

Journal article detailing the manganese-dependent transcriptional regulation mechanisms involving MntR and PerR in Thermus thermophilus HB8.

Biolayer Interferometry for DNA-Protein Interactions v2

Published by

protocols.io

Summary

Preprint describing an updated protocol for using biolayer interferometry to study DNA-protein interactions.

A CsoR family transcriptional regulator, TTHA1953, controls the sulfur oxidation pathway in Thermus thermophilus HB8

Published by

Journal of Biological Chemistry

Summary

Journal article identifying TTHA1953 as a CsoR family transcriptional regulator that controls the sulfur oxidation pathway in Thermus thermophilus HB8.

Using Restriction Endonuclease, Protection, Selection, and Amplification to Identify Preferred DNA-Binding Sequences of Microbial Transcription Factors

Published by

Microbiology Spectrum

Summary

Journal article presenting a method utilizing restriction endonuclease protection, selection, and amplification for identifying preferred DNA-binding sequences of microbial transcription factors.

Identification of the Preferred DNA-Binding Sequence and Transcription Regulatory Network for the Thermophilic Zinc Uptake Regulator TTHA1292

Published by

Journal of Bacteriology

Summary

Journal article detailing the identification of the preferred DNA-binding sequence and transcription regulatory network for the Thermophilic Zinc Uptake Regulator TTHA1292.

Biolayer interferometry for DNA-protein interactions

Published by

PLOS ONE

Summary

Journal article describing the application of biolayer interferometry for the analysis of DNA-protein interactions.

Discovering the DNA-Binding Consensus of the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359

Published by

International Journal of Molecular Sciences

Summary

Journal article focused on discovering the DNA-binding consensus sequence for the Thermus thermophilus HB8 transcriptional regulator TTHA1359.

Biolayer Interferometry for DNA-Protein Interactions v1

Published by

protocols.io

Summary

Preprint detailing a protocol for using biolayer interferometry to study DNA-protein interactions.

Expression and Purification of Thermostable Proteins Expressed in E. coli v1

Published by

protocols.io

Summary

Preprint outlining a protocol for the expression and purification of thermostable proteins from E. coli.

Determination and Dissection of DNA-Binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099

Published by

International Journal of Molecular Sciences

Summary

Journal article investigating and dissecting the DNA-binding specificity of the Thermus thermophilus HB8 transcriptional regulator TTHB099.

Restriction Endonuclease Protection Assays using Infrared-Fluorescent Probes

Published by

protocols.io

Summary

Journal article describing restriction endonuclease protection assays utilizing infrared-fluorescent probes.

General and Genomic DNA-Binding Specificity for the Thermus thermophilus HB8 Transcription Factor TTHB023

Published by

Biomolecules

Summary

Journal article on the general and genomic DNA-binding specificity of the Thermus thermophilus HB8 transcription factor TTHB023.

Identification and Characterization of Preferred DNA-Binding Sites for the Thermus thermophilus HB8 Transcriptional Regulator TTHA0973

Published by

International Journal of Molecular Sciences

Summary

Journal article identifying and characterizing the preferred DNA-binding sites for the Thermus thermophilus HB8 transcriptional regulator TTHA0973.

Design and PCR Synthesis of Infrared Fluorophore-Labeled Modular DNA Probes

Published by

protocols.io

Summary

Journal article detailing the design and PCR synthesis of infrared fluorophore-labeled modular DNA probes.

Electrophoretic Mobility Shift Assays using Infrared-Fluorescent DNA Probes

Published by

protocols.io

Summary

Journal article describing electrophoretic mobility shift assays using infrared-fluorescent DNA probes.

DNA Analysis by Native Polyacrylamide Gel Electrophoresis and Infrared Fluorescence Imaging v2

Published by

preprint

Summary

Preprint on the analysis of DNA using native polyacrylamide gel electrophoresis and infrared fluorescence imaging, version 2.

DNA Analysis by Native Polyacrylamide Gel Electrophoresis and Infrared Fluorescence Imaging v1

Published by

preprint

Summary

Preprint on the analysis of DNA using native polyacrylamide gel electrophoresis and infrared fluorescence imaging, version 1.

Direct Double-Stranded DNA Quantitation from PCR Reactions v1

Published by

preprint

Summary

Preprint describing direct double-stranded DNA quantitation from PCR reactions, version 1.

Direct Double-Stranded DNA Quantitation from PCR Reactions v2

Published by

preprint

Summary

Preprint describing direct double-stranded DNA quantitation from PCR reactions, version 2.

Identification and characterization of preferred DNA-binding sites for the Thermus thermophilus transcriptional regulator FadR

Published by

PLOS ONE

Summary

Journal article identifying and characterizing preferred DNA-binding sites for the Thermus thermophilus transcriptional regulator FadR.

Identification of Preferred DNA-Binding Sites for the Thermus thermophilus Transcriptional Regulator SbtR by the Combinatorial Approach REPSA

Published by

PLOS ONE

Summary

Journal article on the identification of preferred DNA-binding sites for the Thermus thermophilus transcriptional regulator SbtR using the REPSA combinatorial approach.

Lysine deacetylase (KDAC) regulatory pathways: an alternative approach to selective modulation.

Published by

journal-article

Summary

Journal article discussing alternative approaches to selective modulation of lysine deacetylase (KDAC) regulatory pathways.

The Saccharomyces cerevisiae protein Stm1p facilitates ribosome preservation during quiescence

Published by

journal-article

Summary

Journal article investigating how the Saccharomyces cerevisiae protein Stm1p contributes to ribosome preservation during cellular quiescence.

Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer

Published by

journal-article

Summary

Journal article exploring the association of triplex DNA-binding proteins with clinical outcomes in colorectal cancer patients through proteomic analysis.

Molecular mechanism by which the nucleoid occlusion factor, SImA, keeps cytokinesis in check

Published by

journal-article

Summary

Journal article detailing the molecular mechanism by which the nucleoid occlusion factor, SImA, regulates cytokinesis.

Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16

Published by

journal-article

Summary

Journal article investigating the interaction of nucleolin protein with the microprocessor complex and its impact on microRNA 15a and 16 biogenesis.

REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences.

Published by

journal-article

Summary

Journal article describing the REPSA combinatorial approach for identifying preferred drug-DNA binding sequences.

REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences.

Published by

Methods in molecular biology (Clifton, N.J.)

Summary

Journal article outlining the REPSA combinatorial approach for identifying preferred drug-DNA binding sequences within molecular biology methods.

Parthenolide promotes the ubiquitination of MDM2 and activates p53 cellular functions

Published by

journal-article

Summary

Journal article demonstrating how parthenolide promotes MDM2 ubiquitination and activates p53 cellular functions.

Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation

Published by

journal-article

Summary

Journal article investigating how Stm1p modifies the ribosome association of eukaryotic elongation factor 3, impacting translation elongation.

Constitutive and inducible nuclear factor-KB in immortalized normal human bronchial epithelial and non-small cell lung cancer cell lines

Published by

journal-article

Summary

Journal article examining constitutive and inducible nuclear factor-KB activity in immortalized normal human bronchial epithelial and non-small cell lung cancer cell lines.

Parthenolide Specifically Depletes Histone Deacetylase 1 Protein and Induces Cell Death through Ataxia Telangiectasia Mutated

Published by

journal-article

Summary

Journal article demonstrating that parthenolide specifically depletes Histone Deacetylase 1 protein and induces cell death via Ataxia Telangiectasia Mutated pathway.

REPSA: General combinatorial approach for identifying preferred ligand-DNA binding sequences

Published by

journal-article

Summary

Journal article presenting a general combinatorial approach (REPSA) for identifying preferred ligand-DNA binding sequences.

Depletion of histone deacetylase protein: a common consequence of inflammatory cytokine signaling?

Published by

journal-article

Summary

Journal article exploring whether depletion of histone deacetylase protein is a common outcome of inflammatory cytokine signaling.

Depletion of histone deacetylase protein: A common consequence of inflammatory cytokine signaling?

Published by

Cell Cycle

Summary

Journal article investigating if histone deacetylase protein depletion is a common consequence of inflammatory cytokine signaling.

Stm1p, a Ribosome-associated Protein, is Important for Protein Synthesis in Saccharomyces cerevisiae under Nutritional Stress Conditions

Published by

journal-article

Summary

Journal article on the importance of Stm1p, a ribosome-associated protein, for protein synthesis in Saccharomyces cerevisiae under nutritional stress.

Tumour necrosis factor-a depletes histone deacetylase 1 protein through IKK2

Published by

journal-article

Summary

Journal article demonstrating that tumor necrosis factor-alpha depletes histone deacetylase 1 protein via IKK2.

Combinatorial identification of a novel consensus sequence for the covalent DNA-binding polyamide tallimustine

Published by

journal-article

Summary

Journal article describing the combinatorial identification of a novel consensus sequence for the covalent DNA-binding polyamide tallimustine.

Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae

Published by

journal-article

Summary

Journal article investigating the interactions of Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae.

Combinatorial determination of sequence specificity for nanomolar DNA-binding hairpin polyamides

Published by

journal-article

Summary

Journal article on the combinatorial determination of sequence specificity for nanomolar DNA-binding hairpin polyamides.

Identification of preferred actinomycin-DNA binding sites by the combinatorial method REPSA

Published by

journal-article

Summary

Journal article identifying preferred actinomycin-DNA binding sites using the REPSA combinatorial method.

Characterization of a triplex DNA-binding protein encoded by an alternative reading frame of loricrin

Published by

journal-article

Summary

Journal article characterizing a triplex DNA-binding protein encoded by an alternative reading frame of loricrin.

The yeast CDP1 gene encodes a triple-helical DNA-binding protein

Published by

Nucleic Acids Research

Summary

Journal article reporting that the yeast CDP1 gene encodes a triple-helical DNA-binding protein.

The yeast STM1 gene encodes a purine motif triple helical DNA-binding protein

Published by

journal-article

Summary

Journal article demonstrating that the yeast STM1 gene encodes a purine motif triple helical DNA-binding protein.

Differential effects of cyclopolyamines on the stability and conformation of tripler DNA

Published by

Antisense and Nucleic Acid Drug Development

Summary

Journal article investigating the differential effects of cyclopolyamines on the stability and conformation of triplex DNA.

Plasmids for the in vitro analysis of RNA polymerase II-dependent transcription based on a G-free template

Published by

journal-article

Summary

Journal article introducing plasmids for in vitro analysis of RNA polymerase II-dependent transcription using a G-free template.

Characterization of purine-motif triplex DNA-binding proteins in HeLa extracts

Published by

journal-article

Summary

Journal article characterizing purine-motif triplex DNA-binding proteins found in HeLa extracts.

Self-association of G-rich oligodeoxyribonucleotides under conditions promoting purine-motif triplex formation

Published by

Antisense and Nucleic Acid Drug Development

Summary

Journal article investigating the self-association of G-rich oligodeoxyribonucleotides under conditions favoring purine-motif triplex formation.

Multiple rounds of transcription by RNA polymerase II at covalently cross-linked templates

Published by

journal-article

Summary

Journal article describing multiple rounds of transcription by RNA polymerase II on covalently cross-linked templates.

Oligodeoxyribonucleotide length and sequence effects on intramolecular and intermolecular G-quartet formation

Published by

journal-article

Summary

Journal article examining the effects of oligodeoxyribonucleotide length and sequence on intramolecular and intermolecular G-quartet formation.

Effects of chain length modification and bis(ethyl) substitution of spermine analogs on purine-purine-pyrimidine triplex DNA stabilization, aggregation, and conformational transitions

Published by

journal-article

Summary

Journal article analyzing the effects of chain length modification and bis(ethyl) substitution of spermine analogs on triplex DNA stability, aggregation, and conformational changes.

Identification of preferred distamycin-DNA binding sites by the combinatorial method REPSA

Published by

journal-article

Summary

Journal article identifying preferred distamycin-DNA binding sites using the REPSA combinatorial method.

Identification of preferred hTBP DNA binding sites by the combinatorial method REPSA

Published by

journal-article

Summary

Journal article identifying preferred hTBP DNA binding sites using the REPSA combinatorial method.

Transcription factor AP2 is required for expression of the rat transforming growth factor-a gene

Published by

journal-article

Summary

Journal article demonstrating the requirement of transcription factor AP2 for the expression of the rat transforming growth factor-alpha gene.

In vivo persistence of DNA triple helices containing psoralen-conjugated oligodeoxyribonucleotides

Published by

journal-article

Summary

Journal article investigating the in vivo persistence of DNA triple helices containing psoralen-conjugated oligodeoxyribonucleotides.

Sequence specificity of triplex DNA formation: Analysis by a combinatorial approach, restriction endonuclease protection selection and amplification

Published by

journal-article

Summary

Journal article analyzing the sequence specificity of triplex DNA formation using a combinatorial approach, restriction endonuclease protection selection, and amplification.

Indirect use of immobilized metal affinity chromatography for isolation and characterization of protein partners.

Published by

Genetic engineering

Summary

Journal article discussing the indirect use of immobilized metal affinity chromatography for isolating and characterizing protein partners in genetic engineering.

Polyamine effects on purine-purine-pyrimidine triple helix formation by phosphodiester and phosphorothioate oligodeoxyribonucleotides

Published by

Nucleic Acids Research

Summary

Journal article examining polyamine effects on purine-purine-pyrimidine triple helix formation by phosphodiester and phosphorothioate oligodeoxyribonucleotides.

Sp1, USF, and GAL4 activate transcription independently of TFIID-downstream promoter interactions

Published by

Biochimica et Biophysica Acta - Gene Structure and Expression

Summary

Journal article demonstrating that Sp1, USF, and GAL4 activate transcription independently of TFIID-downstream promoter interactions.

Oligodeoxyribonucleotide length and sequence effects on intermodular purine-purine-pyrimidine triple-helix formation

Published by

Nucleic Acids Research

Summary

Journal article investigating the effects of oligodeoxyribonucleotide length and sequence on intermodular purine-purine-pyrimidine triple-helix formation.

Monovalent cation effects on intermolecular purine - purine - pyrimidine triple-helix formation

Published by

Nucleic Acids Research

Summary

Journal article investigating monovalent cation effects on intermolecular purine-purine-pyrimidine triple-helix formation.

An alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2

Published by

Molecular and Cellular Biology

Summary

Journal article reporting that an alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2.

Initiator sequences direct downstream promoter binding by human transcription factor IID

Published by

Biochimica et Biophysica Acta - Gene Structure and Expression

Summary

Journal article showing that initiator sequences direct downstream promoter binding by human transcription factor IID.

Protein-protein interaction studies using immobilized oligohistidine fusion proteins

Published by

journal-article

Summary

Journal article describing protein-protein interaction studies using immobilized oligohistidine fusion proteins.

Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain

Published by

Gene

Summary

Journal article detailing a single-step purification method for bacterially expressed polypeptides with an oligo-histidine domain.

In vitro inhibition of c-myc transcription by mithramycin

Published by

Biochemical and Biophysical Research Communications

Summary

Journal article on the in vitro inhibition of c-myc transcription by mithramycin.

A rapid method for the purification of deprotected oligodeoxynucleotides

Published by

Nucleic Acids Research

Summary

Journal article presenting a rapid method for purifying deprotected oligodeoxynucleotides.

DNA-binding and transcriptional properties of human transcription factor TFIID after mild proteolysis

Published by

Molecular and Cellular Biology

Summary

Journal article on the DNA-binding and transcriptional properties of human transcription factor TFIID following mild proteolysis.

Stability of transcription complexes on class II genes

Published by

Molecular and Cellular Biology

Summary

Journal article investigating the stability of transcription complexes on class II genes.

Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei

Published by

Journal of Biological Chemistry

Summary

Journal article detailing the complete purification and identification of multiple forms of human gene-specific transcription factor USF from HeLa cell nuclei.

Physical analysis of transcription preinitiation complex assembly on a class II gene promoter

Published by

Science

Summary

Journal article providing a physical analysis of transcription preinitiation complex assembly on a class II gene promoter.

Multiple proteins bind to VA RNA genes of adenovirus Type 2

Published by

Molecular and Cellular Biology

Summary

Journal article identifying multiple proteins that bind to VA RNA genes of adenovirus Type 2.

Novobiocin interferes with the binding of transcription factors TFIIA and TFIIIC to the promoters of class III genes

Published by

Nucleic Acids Research

Summary

Journal article demonstrating that Novobiocin interferes with the binding of transcription factors TFIIA and TFIIIC to class III gene promoters.

Two forms of transcription factor TFIIIC in extracts from HeLa cells

Published by

Nucleic Acids Research

Summary

Journal article identifying two forms of transcription factor TFIIIC in extracts from HeLa cells.

Echinomycin binding sites on DNA

Published by

Science

Summary

Journal article identifying echinomycin binding sites on DNA.

Chromomycin, mithramycin, and olivomycin binding sites on heterogeneous deoxyribonucleic acid. Footprinting with (methidiumpropyl-EDTA)iron(II)

Published by

Biochemistry

Summary

Journal article detailing the binding sites of chromomycin, mithramycin, and olivomycin on heterogeneous deoxyribonucleic acid using methidiumpropyl-EDTA-iron(II) footprinting.

Methidiumpropyl-EDTA-Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA

Published by

journal-article

Summary

Journal article comparing small molecule binding site sizes on DNA reported by Methidiumpropyl-EDTA-Fe(II) and DNase I footprinting.

Footprinting with MPE-Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA

Published by

Cold Spring Harbor Symposia on Quantitative Biology

Summary

Journal article presenting complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA using MPE-Fe(II) footprinting.

Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA-Fe(II)

Published by

Proceedings of the National Academy of Sciences of the United States of America

Summary

Journal article mapping distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA using DNA cleavage-inhibition patterns with methidiumpropyl-EDTA-Fe(II).

Skills

Research & Development

Ligand-nucleic acid interactions, Iterative selection methods, Transcriptional regulation, Extremophiles, Nonradioactive assay development, Small molecule-DNA interactions, Eukaryotic transcription mechanisms, Combinatorial selection methods, Reverse-genetic approach, DNA-protein interactions, Restriction Endonuclease Protection Assays (REPSA), Biolayer Interferometry, Protein Expression and Purification, PCR Synthesis, Electrophoretic Mobility Shift Assays (EMSA), DNA Quantitation, Proteomics, Ribosome homeostasis, Nucleic Acid Analysis, Spectropolarimetry, Tumor Biology, Molecular & Cellular Oncology.

Molecular Biology Techniques

DNA-binding assays, RNA polymerase II transcription, Oligodeoxyribonucleotide synthesis, Immobilized metal affinity chromatography, G*G multiplex nucleic acids, Restriction endonucleases, Infrared-fluorescent probes, Native Polyacrylamide Gel Electrophoresis, Infrared Fluorescence Imaging, Histone deacetylase regulation, MicroRNA biogenesis.

Academic Leadership & Education

Professor, Associate Professor, Curriculum Development, Undergraduate Mentorship, Graduate Student Mentorship, Postdoctoral Fellow Mentorship, Scientific Writing, Peer Review, Grant Writing, Grant Management, Departmental Service.

Scientific Domains

Biochemistry, Molecular Biology, Chemistry, Chemical Engineering, Microbiology, Genetics, Cell Biology, Cancer Research.

Data Analysis & Interpretation

Experimental Design, Data Analysis, Scientific Reporting.