Rubén G. Contreras

About

Doctor en Fisiología Celular y Molecular, Cinvestav 1991; Postdoctorado en Centro de Neurobiología, Hamburgo, Alemania 1992. Interesado en la Polaridad, contactos celulares y permeabilidad epitelial, Na/K-ATPasa como receptor y molécula de adhesión, y el papel de la ouabaina como hormona reguladora epitelial. Demostré que el ion calcio dispara la formación, ensamble y sellado de las uniones oclusoras e induce la expresión polarizada de la Na/K-ATPasa fundamentales en los epitelios. Demostré también que la expresión polarizada de la Na/K-ATPasa involucra un mecanismo que la quita de los sitios de la membrana en donde no debe estar. Además, demostré que la ouabaína, una substancia que hoy se considera una hormona reguladora de la presión arterial, entre otras funciones, afecta drásticamente los contactos celulares a través de vías metabólicas que inducen la eliminación de las moléculas de adhesión de la membrana celular, y a concentraciones semejantes a la hormonal, aumenta la adhesion, comunicación y migración celular. Entre las vías metabolicas de control, describí una nueva que involucra al factor de transcripcion STAT3. Tengo intrés en investigar los mecanismos de control de la adhesión por señales del ambiente e investigué cómo el factor de crecimiento epidermal induce la diferenciación de las células epiteliales a estados más herméticos. Dado que muchas enfermedades autoinmunes se generan por un aumento de la permeabilidad del epitelio intestinal, la investigación sobre los efectos de este factor arrojan luz sobre los mecanismos patogénicos de estas enfermedades y plantea posibles tratamientos. Dado también que muchas de las enfermedades comienzan con la interaccion del agente patogeno con un epitelio, los resultados que hemos publicado ayudan a entender los mecanismos celulares de infecciones y el cáncer

Work

Cinvestav (Centro de Investgación y de Estudios Avanzados del Instituto Politécnico Nacional)

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Investigador CINVESTAV 3C

Mexico

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Education

Universitätsklinikum Hamburg-Eppendorf Zentrum für Molekulare Neurobiologie Hamburg

Germany
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Posdoc

CINVESTAV Departamento de Fisiologia Biofisica y Neurociencias

Mexico
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Doctor en Ciencias especialidad de Fisiología

Universidad Nacional Autónoma de México

Mexico
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Maestría en Ciencias, especialidad en Biología Molecular

Universidad Autónoma del Estado de Morelos

Mexico
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Licenciado en Biología, especialidad en Ecología Humana

Publications

Cardiac Glycosides: From Natural Defense Molecules to Emerging Therapeutic Agents

Biomolecules

journal-article

Cardiac Glycosides: From Natural Defense Molecules to Emerging Therapeutic Agents

Biomolecules

journal-article

Na+/K+-ATPase: More than an Electrogenic Pump

International Journal of Molecular Sciences

journal-article

Na+/K+-ATPase: More than an Electrogenic Pump

International Journal of Molecular Sciences

journal-article

Ouabain promotes claudin-1, -2, and -4 autophagic degradation through oxidative stress and AMPK activation in MDCK cells

Autophagy Reports

journal-article

Ouabain promotes claudin-1, -2, and -4 autophagic degradation through oxidative stress and AMPK activation in MDCK cells

Autophagy Reports

journal-article

ROS Modulation on Apical Junctional Complex

Handbook of Oxidative Stress in Cancer: Mechanistic Aspects

book-chapter

Chloroquine disrupts zinc storage granules in primary Malpighian tubule cells of Drosophila melanogaster

Metallomics

journal-article

Chloroquine disrupts zinc storage granules in primary Malpighian tubule cells of Drosophila melanogaster

Metallomics

journal-article

Relationship Between ROS, Autophagy, and Cancer

Handbook of Oxidative Stress in Cancer: Mechanistic Aspects

book-chapter

In vitro Evaluation of Programmed Cell Death in the Immune System of Pacific Oyster Crassostrea gigas by the Effect of Marine Toxins

Frontiers in Immunology

journal-article

In vitro Evaluation of Programmed Cell Death in the Immune System of Pacific Oyster Crassostrea gigas by the Effect of Marine Toxins

Frontiers in Immunology

journal-article

In vitro Evaluation of Programmed Cell Death in the Immune System of Pacific Oyster Crassostrea gigas by the Effect of Marine Toxins

Frontiers in Immunology

journal-article

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

Neuroscience

journal-article

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

Neuroscience

journal-article

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

Neuroscience

journal-article

Ouabain Modulates the Adherens Junction in Renal Epithelial Cells

Cellular Physiology Biochemistry

journal-article

Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

The Journal of Membrane Biology

journal-article

Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

Journal of Membrane Biology

journal-article

Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

Journal of Membrane Biology

journal-article

Correction to: Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

The Journal of Membrane Biology

journal-article

Correction to: Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity (The Journal of Membrane Biology, (2019), 252, 6, (549-559), 10.1007/s00232-019-00066-5)

Journal of Membrane Biology

journal-article

Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity (vol 252, pg 549, 2019)

Journal of Membrane Biology

journal-article

Ouabain modulates the adherens junction in renal epithelial cells

Cellular Physiology and Biochemistry

journal-article

HPV16-E6 Oncoprotein Activates TGF-β and Wnt/β-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model

BioMed Research International

journal-article

Involvement of Src signaling in the synergistic effect between cisplatin and digoxin on cancer cell viability

Journal of Cellular Biochemistry

journal-article

Involvement of Src signaling in the synergistic effect between cisplatin and digoxin on cancer cell viability

Journal of Cellular Biochemistry

journal-article

Differential homologous desensitization of the human histamine H3 receptors of 445 and 365 amino acids expressed in CHO-K1 cells

Neurochemistry International

journal-article

HPV16-E6 Oncoprotein Activates TGF- β and Wnt/ β -Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model

BioMed Research International

journal-article

Lysosomal Degradation of Junctional Proteins

Lysosomes - Associated Diseases and Methods to Study Their Function

book-chapter

The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemneß Transcriptional Profile in Vivo

BioMed Research International

journal-article

The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional Profile In Vivo

BioMed Research International

journal-article

Ouabain Modulates the Distribution of Connexin 43 in Epithelial Cells

Cellular Physiology and Biochemistry

journal-article

Respuesta inmunológica ante la infección por el virus del papiloma humano

Aspectos Inmunitarios de mucosas y piel

book-chapter

EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells

Journal of Cellular Physiology

journal-article

The Polarized distribution of the Na+, K+-ATPase

Cell Polarity 1: Biological Role and Basic Mechanisms

book

The Polarized Distribution of the Na+,K+-ATPase

Cell Polarity 1

book-chapter

The Polarized Distribution of the Na+, K+-ATPase

CELL POLARITY 1: BIOLOGICAL ROLE AND BASIC MECHANISMS

journal-article

EGF regulates claudin-2 and -4 expression through STAT3 and Src in MDCK cells

J. Cell. Physiol.

journal-article

Ouabain induces endocytosis and degradation of tight junction proteins through ERK1/2-dependent pathways

Experimental Cell Research

journal-article

Ouabain induces endocytosis and degradation of tight junction proteins through ERK1/2-dependent pathways

Experimental Cell Research

journal-article

Anterior and intermediate pituitary tissues express claudin 4 in follicle stellate cells and claudins 2 and 5 in endothelial cells

Cell and Tissue Research

journal-article

21-benzylidene digoxin: A proapoptotic cardenolide of cancer cells that up-regulates Na,K-ATPase and epithelial tight junctions

PLoS ONE

journal-article

Ouabain increases gap junctional communication in epithelial cells

Cellular Physiology and Biochemistry

journal-article

Apoptosis of hemocytes from lions-paw scallop Nodipecten subnodosus induced with paralyzing shellfish poison from Gymnodinium catenatum

Immunobiology

journal-article

Apoptosis of hemocytes from lions-paw scallop Nodipecten subnodosus induced with paralyzing shellfish poison from Gymnodinium catenatum

Immunobiology

journal-article

Accumulation, biotransformation, histopathology and paralysis, in the Pacific calico scallop Argopecten ventricosus, by the effect of the dinoflagellate Gymnodinium catenatum and its paralyzing toxins

Journal of Marine Drugs

journal-article

The Na +-K +-ATPase as self-adhesion molecule and hormone receptor

American Journal of Physiology - Cell Physiology

journal-article

The E6 oncoprotein from HPV16 enhances the canonical Wnt/β-catenin pathway in skin epidermis in vivo

Molecular Cancer Research

journal-article

Ouabain modulates cell contacts as well as functions that depend on cell adhesion

Methods in Molecular Biology

book

Ouabain modulates ciliogenesis in epithelial cells

Proceedings of the National Academy of Sciences of the United States of America

journal-article

Histología y citología de los epitelios

BIOLOGÍA Y CULTIVO DE LOS MOLUSCOS PECTÍNIDOS DEL GÉNERO Nodipecten

book-chapter

Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

Permeability Barrier

other

Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

Permeability Barrier: Methods and Protocols

journal-article

Effects of induced paralysis on hemocytes and tissues of the giant lions-paw scallop by paralyzing shellfish poison

Marine Biology

journal-article

The polarized distribution of Na+,K+-ATPase: Role of the interaction between β subunits

Molecular Biology of the Cell

journal-article

Effects of induced paralysis on hemocytes and tissues of the giant lions-paw scallop by paralyzing shell poison

Marine Biology

journal-article

Ouabain modulates epithelial cell tight junction

Proceedings of the National Academy of Sciences of the United States of America

journal-article

Control of tight junctional sealing: Roles of epidermal growth factor and prostaglandin E2

American Journal of Physiology - Cell Physiology

journal-article

Tight junction and polarity interaction in the transporting epithelial phenotype

Biochimica et Biophysica Acta - Biomembranes

journal-article

Tight junction and polarity interaction in the transporting epithelial phenotype

Biochimica et Biophysica Acta (BBA) - Biomembranes

journal-article

Absorption of paralyzing toxins from the dinoflagellate Gymnodinium catenatum in giant lions-paw scallop Nodipecten subnodosus

Journal of Shellfish Research

journal-article

New Diseases Derived or Associated with the Tight Junction

Archives of Medical Research

journal-article

Control of tight junctional sealing: Role of epidermal growth factor

American Journal of Physiology - Renal Physiology

journal-article

Evolution of the Transporting Epithelium Phenotype

Tight Junctions

other

Na+,K+-ATPase and hormone ouabain: New roles for an old enzyme and an old inhibitor

Cellular and Molecular Biology

journal-article

Contacts and cooperation between cells depend on the hormone ouabain

Proceedings of the National Academy of Sciences of the United States of America

journal-article

Regulation of tTight junctions´functional integrity: role of a urinary factor, lipids and ouabain

Tight Junctions

book-chapter

Sodium/potasium ATPase (Na+, K+-ATPase) and ouabain/related cardiac glycosides: A new paradigm for development of anti- breast cancer drugs?

Breast Cancer Research and Treatment

journal-article

Na+,K+-ATPase, the star of epithelial net transports: what do we know about its own polarizad distribution.

Physiological Mini-Reviews

journal-article

Signaling along the P→A mechanism involves changes in phosphorylation state and localization of cell adhesion proteins

The Journal of General Physiology

journal-article

The polarized expression of Na+,K+-ATPase depends on the association between beta-subunits located in neighboring cells.

The Journal of General Physiology

journal-article

The polarized expression of Na+,K+-ATPase in epithelia depends on the association between β-subunits located in neighboring cells

Molecular Biology of the Cell

journal-article

Ouabain binding to Na+,K+-ATPase relaxes cell attachment and sends a specific signal (NACos) to the nucleus

Journal of Membrane Biology

journal-article

Cell adhesion, polarity, and epithelia in the dawn of metazoans

Physiological Reviews

journal-article

Inhibitors of glycosphingolipid biosynthesis reduce transepithelial electrical resistance in MDCK I and FRT cells

American Journal of Physiology - Cell Physiology

journal-article

La membrana celular

Biología Celular y Molecular

book-chapter

Membrane targeting

Progress in Biophysics and Molecular Biology

journal-article

Functional Analysis of the Tight Junction

Cell-Cell Interactions

book-chapter

Regulation of the tight junction (TJ) through a peptide contained in the urine

Molecular Biology of the Cell

journal-article

The pump→attachment mechanism:: Signalling

Molecular Biology of the Cell

journal-article

Tight junctions are sensitive to peptides eliminated in the urine

Journal of Membrane Biology

journal-article

The pump→attachment mechanism:: Pumping should be stopped

Molecular Biology of the Cell

journal-article

Functional analysis of tight junctions

Cell-Cell Interactions

book-chapter

Cytochalasin-D retards sperm incorporation deep into the egg cytoplasm but not membrane fusion with the egg plasma membrane

Molecular Reproduction and Development

journal-article

E-cadherin and tight junctions between epithelial cells of different animal species

Pflugers Archiv European Journal of Physiology

journal-article

Biogenesis of epithelial polarity and tight junctions

Tight Junctions

book-chapter

Biogenesis of Epithelial Polarity and Tight Junctions

Tight Junctions

other

Biogenesis of epithelial polarity and tight junctions

Tight Junctions

book-chapter

Cell-cell contacts and the assembly of tight junctions

Molecular Biology of the Cell

journal-article

Exocytosis of a 60 kDa protein (Calreticulin) from activated hamster oocytes

Molecular Reproduction and Development

journal-article

The polarized distribution of Na+, K+-ATPase and active transport across epithelia

Journal of Membrane Biology

journal-article

Molecular physiology and pathophysiology of tight junctions - I. Biogenesis of tight junctions and epithelial polarity

American Journal of Physiology-Gastrointestinal and Liver Physiology

journal-article

Molecular physiology and pathophysiology of tight junctions. I. Biogenesis of tight junctions and epithelial polarity

American Journal of Physiology - Gastrointestinal and Liver Physiology

journal-article

Molecular characterization of the tight junction protein ZO-1 in MDCK cells

Experimental Cell Research

journal-article

Na+, K+-ATPase and cell attachment:: mechanisms

Molecular Biology of the Cell

journal-article

Na+,K+-ATPase and cell attachment:: signaling.

Molecular Biology of the Cell

journal-article

Na+,K+-ATPase, phosphorylation, and cell attachment

The FASEB Journal

journal-article

Relationship between Na+,K+-ATPase and cell attachment

Journal of Cell Science

journal-article

Relationship between Na+,K+-ATPase and cell attachment

Journal of Cell Science

journal-article

ROLE OF TIGHT JUNCTIONS IN ESTABLISHING AND MAINTAINING CELL POLARITY

Annu. Rev. Physiol.

journal-article

Role of tight junctions in establishing and maintaining cell polarity

Annual Review of Physiology

book

Relationship between Na+,K+-ATPase tyrosine phosphorylation and cell attachment

Molecular Biology of the Cell

journal-article

Tight junctions and the experimental modifications of lipid content

Journal of Membrane Biology

journal-article

Na+, K,-ATPase and cell attachment

FASEB Journal

journal-article

Cytoplasmic DNA in Entamoeba histolytica: its biological significance

Arch. Med. Res.

journal-article

Effect of calcium and cell-cell contacts on the making of tight junctions

Molecular Biology of the Cell

journal-article

Na+, K-+-ATPase and cell attachment

The FASEB Journal

journal-article

The role of E-cadherin in the polarization of epithelial cells

Molecular Biology of the Cell

journal-article

Epithelial Transport

Epithelial Polarity

book-chapter

Molecular characterization of tight junction protein ZO-1 in MDCK cells

Molecular Biology of the Cell

journal-article

Morphogenesis of the epithelial cell transporting phenotype: Synthesis and distribution of ion channels

Brazilian Journal of Medical and Biological Research

journal-article

A NOVEL TYPE OF CELL-CELL COOPERATION BETWEEN EPITHELIAL-CELLS

Journal of Membrane Biology

journal-article

A novel type of cell-cell cooperation between epithelial cells

The Journal of Membrane Biology

journal-article

EXPRESSION OF POTASSIUM CHANNELS IN EPITHELIAL-CELLS DEPENDS ON CALCIUM-ACTIVATED CELL-CELL CONTACTS

Journal of Membrane Biology

journal-article

Expression of potassium channels in epithelial cells depends on calcium-activated cell-cell contacts

The Journal of Membrane Biology

journal-article

NA+, K+-PUMP AND CELL ATTACHMENT

The FASEB Journal

journal-article

OUABAIN RESISTANCE OF THE EPITHELIAL-CELL LINE (MA104) IS NOT DUE TO LACK OF AFFINITY OF ITS PUMPS FOR THE DRUG

Journal of Membrane Biology

journal-article

Ouabain resistance of the epithelial cell line (Ma104) is not due to lack of affinity of its pumps for the drug

The Journal of Membrane Biology

journal-article

Extracellular and intracellular regulation of junctional assembly in epithelial cells

Molecular mechanisms of epithelial cell junctions: from development to disease

book-chapter

Extracellular and intracellular regulation of junctional assembly in epithelial cells

Molecular Mechanisms of Epithelial Cell Junctions

book-chapter

THE MAKING OF A TIGHT JUNCTION

Journal of Cellular Biochemistry

journal-article

The making of a tight junction

Journal of Cell Science

journal-article

The paracellular pathway

Biological Barriers to Protein Delivery

book-chapter

The Paracellular Pathway

Biological Barriers to Protein Delivery

other

CALCIUM INTERACTS AT THE EXTRACELLULAR FACE OF THE PLASMA-MEMBRANE OF EPITHELIAL (MDCK) CELLS TO TRIGGER TIGHT JUNCTIONS FORMATION

Molecular Biology of the Cell

journal-article

Calcium and the tight junctions

Tight Junctions

book-chapter

IDENTIFICATION OF A FACTOR IN URINE THAT INCREASES TRANSEPITHELIAL ELECTRICAL-RESISTANCE IN MDCK CELLS

Molecular Biology of the Cell

journal-article

INTERACTION OF CALCIUM WITH PLASMA-MEMBRANE OF EPITHELIAL (MDCK) CELLS DURING JUNCTION FORMATION

The American Journal of Physiology

journal-article

Interaction of calcium with plasma membrane of epithelial (MDCK) cells during junction formation

American Journal of Physiology - Cell Physiology

journal-article

Polarized distribution of chloride channels in epithelial cells (MDCK)

Cellular Physiology and Biochemistry

journal-article

Assembly and sealing of tight junctions: Possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin

The Journal of Membrane Biology

journal-article

ROLE OF CALCIUM IN TIGHT JUNCTION FORMATION BETWEEN EPITHELIAL-CELLS

The American Journal of Physiology

journal-article

Role of calcium in tight junction formation between epithelial cells

American Journal of Physiology - Cell Physiology

journal-article

Development and alteration of polarity

Annual Review of Physiology

book

Tight Junction: Barrier Between Higher Organisms and Environment

Physiology

journal-article

REPOLARIZATION OF NA+-K+ PUMPS DURING ESTABLISHMENT OF EPITHELIAL MONOLAYERS

American Journal of Physiology

journal-article

Repolarization of Na+-K+ pumps during establishment of epithelial monolayers

American Journal of Physiology - Cell Physiology

journal-article

Epithelial tight junctions

American Review of Respiratory Disease

journal-article

Tight Junctions

Critical Reviews of Anatomical Sciences

journal-article

El riñón y la homeostasis orgánica

Nefrología Pediátrica

book-chapter