Chia-Ying Huang

About

I am Taiwanese and grew up in a family of nine. I earned my PhD in Microbiology and Immunology from National Yang-Ming University, Taiwan, in June 2012. My doctoral research, co-advised by Prof. Chi-Huey Wong and Dr. Che Ma at the Genomic Research Center, Academia Sinica, focused on the characterization and 3D structural determination of a bacterial transglycosylase protein. I have one year of experience in bacterial classification from Dr. Tsai-Ling Yang Lauderdale's lab at the National Health Research Institutes, Taiwan, and three years of experience in in meso in situ serial crystallography (IMISX) from Prof. Martin Caffrey's lab at Trinity College Dublin, Ireland. Currently, I am a scientist in Drs. May Sharpe and Meitian Wang’s group at the Swiss Light Source. My research interests center on the X-ray crystallography of membrane and soluble proteins, with recent work focusing on FFCS and temperature-resolved X-ray crystallography. I have collaborated with beamline scientists at various synchrotron facilities, including KEK, Spring-8, APS, DLS, ESRF, and SLS. Additionally, I have extensive experience in protein purification and functional assays of bacterial membrane proteins, both in vivo and in vitro. I am organized, hardworking, and quick to acquire new knowledge and skills.

Work

Paul Scherrer Institut/Swiss Light Source
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Scientist

Switzerland

Paul Scherrer Institut/Swiss Light Source
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PostDoc Research Fellow

Switzerland

Trinity College Dublin
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PostDoc Research Fellow

Ireland

National Health Research Institutes
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PostDoc Research Fellow

Taiwan (Province of China)

Education

National Yang-Ming University
Taiwan (Province of China)

Ph. D

National Changhua University of Education
Taiwan (Province of China)

Master's degree

National Changhua University of Education
Taiwan (Province of China)

Bachelor's degree

Publications

Swiss consortium in macromolecular X-ray crystallography structure determination for academic researchers: EPFL, UNIL, UNIGE and SLS-MX joi

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European Synchrotron Radiation Facility

Summary

data-set

Swiss consortium in macromolecular X-ray crystallography structure determination for academic researchers: EPFL, UNIL, UNIGE and SLS-MX joi

Published by

European Synchrotron Radiation Facility

Summary

data-set

Swiss consortium in macromolecular X-ray crystallography structure determination for academic researchers: EPFL, UNIL, UNIGE and SLS-MX joi

Published by

European Synchrotron Radiation Facility

Summary

data-set

Swiss consortium in macromolecular X-ray crystallography structure determination for academic researchers: EPFL, UNIL, UNIGE and SLS-MX joi

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European Synchrotron Radiation Facility

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data-set

TEST proposal for software developements

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European Synchrotron Radiation Facility

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data-set

Experimental Protein-Ligand Affinity Data for CASP16 Pharmaceutical Ligand Challenge

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Zenodo

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data-set

Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography

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IUCrJ

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Cryo2RT: a high-throughput method for room-temperature macromolecular crystallography from cryo-cooled crystals

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Acta Crystallographica Section D Structural Biology

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HEIDI: an experiment-management platform enabling high-throughput fragment and compound screening

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Acta Crystallographica Section D Structural Biology

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Fragment-based screening targeting an open form of the SARS-CoV-2 main protease binding pocket

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Acta Crystallographica Section D Structural Biology

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Fragment-based screening targeting an open form of the SARS-CoV-2 main protease binding pocket

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preprint

Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein N -acyltransferase

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Science Advances

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SDU – software for high-throughput automated data collection at the Swiss Light Source

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Journal of Synchrotron Radiation

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Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome

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Nature Communications

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Probing ligand binding of endothiapepsin by `temperature-resolved' macromolecular crystallography

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Acta Crystallographica Section D Structural Biology

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Fast fragment- and compound-screening pipeline at the Swiss Light Source

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Acta Crystallographica Section D Structural Biology

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Versatile microporous polymer-based supports for serial macromolecular crystallography

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Acta Crystallographica Section D Structural Biology

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Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis

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Nature Communications

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The crystal structure of bromide-bound GtACR1 reveals a pre-activated state in the transmembrane anion tunnel

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eLife

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The Crystal Structure of Bromide-BoundGtACR1 Reveals a Pre-Activated State in the Transmembrane Anion Tunnel

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preprint

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

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IUCrJ

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3D-printed holders for in meso in situ fixed-target serial X-ray crystallography

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Journal of Applied Crystallography

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Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR

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Journal of the American Chemical Society

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Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin

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Nature Communications

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Crystal structure of a natural light-gated anion channelrhodopsin

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eLife

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Automated data collection and real-time data analysis suite for serial synchrotron crystallography

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Journal of Synchrotron Radiation

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Crystal Structure of Undecaprenyl-Pyrophosphate Phosphatase and its Role in Peptidoglycan Biosynthesis

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Nature Communicationsvolume

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A common antagonistic mechanism for class A GPCRs revealed by the structure of the human 5-HT1B serotonin receptor bound to an antagonist

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Cell discovery

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Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis

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Nature Communication

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In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.

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Acta crystallographica. Section D, Structural biology

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Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals

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Journal of Applied Crystallography

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In meso in situ serial X-ray crystallography of soluble and membrane proteins.

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Acta crystallographica. Section D, Biological crystallography

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Epidemiology and molecular characterization of macrolide-resistant Streptococcus pyogenes in Taiwan.

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Journal of clinical microbiology

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Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism.

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Proceedings of the National Academy of Sciences of the United States of America

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Effect of the Peptide Moiety of Lipid II on Bacterial Transglycosylase

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Angewandte Chemie International Edition

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